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实时荧光定量PCR(RT-FQ-PCR)是一种用于检测DNA或RNA的技术，能够实时监测PCR反应过程中的荧光信号，可以精确定量目标核酸的数量。RT-FQ-PCR在医学、生物学和疾病诊断中具有广泛的应用价值。例如，在COVID-19检测中，RT-FQ-PCR可以快速准确地检测病毒核酸，提供重要的诊断依据。另外，RT-FQ-PCR还可以用于研究基因表达、病毒载量和细菌数量等领域。总的来说，RT-FQ-PCR技术的广泛应用使得科学家们能够更准确地进行核酸定量和表达分析，为疾病诊断和科研工作提供了有力支持。
实时荧光定量PCR(RT-FQ-PCR),RT-FQ-PCR
1)RT-FQ-PCR实时荧光定量PCR(RT-FQ-PCR)
2)quantitative real-time PCR(Q-RT-PCR)荧光实时定量PCR(Q-RT-PCR)
3)FQ-PCR荧光定量PCR(FQ-PCR)
4)real-time fluorescent quantitative RT-PCR实时荧光定量RT-PCR
1.Expressions of bone metastasis genes in nasopharyngeal carcinoma byreal-time fluorescent quantitative RT-PCR;实时荧光定量RT-PCR检测鼻咽癌骨转移相关基因表达的研究
2.Establishment of areal-time fluorescent quantitative RT-PCR assay for the detection of chicken IFN-γ gene;鸡γ-干扰素实时荧光定量RT-PCR检测方法的建立
3.Methods Usingreal-time fluorescent quantitative RT-PCR to analyze the expressions of DNMT1,DNMT3A and DNMT3B in normal liver cell line,pericacinoma cell line and hepatocellular carcinoma cell line respectively.方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。
英文短句/例句
1.Investigation of Expression of CD109, RFP and BRDT Genes Using Real-Time Fluorescence Quantitative RT-PCR;实时荧光定量RT-PCR检测CD109、RFP和BRDT基因表达
2.The mRNA expression of Musashi-1 gene in clonic cancer by quantitative real-time fluorescence RT-PCR实时荧光定量RT-PCR检测结肠癌中Musashi-1 mRNA的表达
3.Expression of CK19-mRNA in Peripheral Blood of Lung Cancer Patients by Real-time Fluorescent Quantitative RT-PCR and Its Clinical Significance;实时荧光定量RT-PCR检测肺癌外周血中CK19mRNA的表达及临床意义
4.Detection of iASPP mRNA in Hepatocellular Carcinoma and Hepatoma Cell Lines by Fluorescence Quantitative RT-PCR.;实时荧光定量RT-PCR检测肝癌及人肝癌细胞株iASPP mRNA
5.Development and Application of a One-step Real-time TaqMan RT-PCR Assay to Detect Duck Hepatitis Virus Type 1实时荧光定量RT-PCR检测1型鸭病毒性肝炎方法的建立和应用
6.Detection and Clinical Significance of hPMS2 mRNA in Gastric Cancer Using Real-time Fluorescent Quantitative RT-PCR实时荧光定量RT-PCR对胃癌组织中hPMS2 mRNA的检测及其临床意义
7.Establishment of a real-time fluorescent quantitative RT-PCR assay for detection of chicken IL-1β,IL-2 and IL-6 genes鸡IL-1β和IL-2及IL-6基因实时荧光定量RT-PCR检测方法的建立
8.Detection of FOXP3 mRNA expression in human peripheral blood mononuclear cells by real-time fluorescent quantitative RT-PCR实时荧光定量RT-PCR检测人外周血单个核细胞中FOXP3 mRNA的表达
9.Development of Real- Time Quantitative RT- PCR for the Detection of Dengue Virus登革热病毒RNA实时荧光定量RT-PCR检测方法的建立
10.The detection of BMI-1 mRNA in leukemic cells with real-time fluorescent quantitative reverse transcription-polymerase chain reaction实时荧光定量RT-PCR检测白血病细胞中BMI-1 mRNA的表达及其意义
11.Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitive RT-PCR实时荧光定量RT-PCR检测肝癌组织中miR-122a及miR-224的表达
12.Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer实时荧光定量RT-PCR分析非小细胞肺癌SATB1的表达和临床病理意义
13.Establishment of a real-time fluorescent quantitative RT-PCR assay for detection of chicken IFN-α,IFN-β and IFN-γ genes鸡IFN-α和IFN-β及IFN-γ基因实时荧光定量RT-PCR检测方法的建立
14.Quantification of enterovirus 71 by real-time fluorescence quantitative RT-PCR in hospitalized children with hand-foot-mouth disease实时荧光定量RT-PCR法检测手足口病患儿大便标本中肠道病毒71型
15.Development of a real-time fluorescent quantitative RT-PCR for detection of infectious bursal disease virus鸡传染性法氏囊病病毒实时荧光定量RT-PCR检测方法的建立
16.Molecular diagnostic index for hepatocellular carcinoma using real-time fluorescent quantitation RT-PCR: a pilot study应用实时荧光定量RT-PCR法建立肝癌分子诊断指数
17.Application of real-time fluorescent RT-PCR for quantitative detection of SARS-Coronavirus实时荧光RT-PCR在SARS病毒定量检测中的应用
18.Development of a Real-Time TaqMan RT-PCR Assay for the Detection of Foot-and-Mouth Disease Virus口蹄疫病毒实时TaqMan荧光定量RT-PCR检测方法的建立
相关短句/例句
quantitative real-time PCR(Q-RT-PCR)荧光实时定量PCR(Q-RT-PCR)
3)FQ-PCR荧光定量PCR(FQ-PCR)
4)real-time fluorescent quantitative RT-PCR实时荧光定量RT-PCR
1.Expressions of bone metastasis genes in nasopharyngeal carcinoma byreal-time fluorescent quantitative RT-PCR;实时荧光定量RT-PCR检测鼻咽癌骨转移相关基因表达的研究
2.Establishment of areal-time fluorescent quantitative RT-PCR assay for the detection of chicken IFN-γ gene;鸡γ-干扰素实时荧光定量RT-PCR检测方法的建立
3.Methods Usingreal-time fluorescent quantitative RT-PCR to analyze the expressions of DNMT1,DNMT3A and DNMT3B in normal liver cell line,pericacinoma cell line and hepatocellular carcinoma cell line respectively.方法用实时荧光定量RT-PCR方法分析正常肝细胞系、肝癌癌旁细胞系及肝癌癌细胞系中DNMT1、DNMT3A、DNMT3B的表达谱,以及DNMT3B表达抑制后在肝癌细胞系SMMC-7721中出现表达上调的与肿瘤发生相关的基因PDCD4和MBD3的表达。
5)Real-time fluorescence quantitative RT-PCR实时荧光定量RT-PCR
1.Quantification of enterovirus 71 by real-time fluorescence quantitative RT-PCR in hospitalized children with hand-foot-mouth disease实时荧光定量RT-PCR法检测手足口病患儿大便标本中肠道病毒71型
2.Methods After treatment of cultured 16HBE cells with NiSO_4 or NiSO_4 adding FJD drug serum,the variation of the free radicals and 8-oxo-dGTPase expression were detected by laser scanning confocal microscopy(LSCM) and real-time fluorescence quantitative RT-PCR,respectively.方法体外培养的16HBE细胞分别经NiSO4暴露及NiSO4加扶正解毒汤含药血清共同处理后,采用激光扫描共聚焦显微镜(LSCM)及实时荧光定量RT-PCR检测自由基含量及8-oxo-dGTPase表达的变化。
3.ConclusionReal-time fluorescence quantitative RT-PCR used to detect the expression of .目的建立实时荧光定量RT-PCR方法检测SurvivinmRNA的表达。
6)real time RT-PCR实时荧光定量RT-PCR
1.One step RT-PCR technique and Real time RT-PCR technique are applied in evaluating the correlation between rate of mRNA degradation in SD rats and postmortem intervals, especially in advanced stage PMI.目的：本研究从基因库中筛选出两种稳定表达于不同个体与组织细胞中的看家基因GAPDH和β-actin，采用实时荧光定量RT-PCR技术研究大鼠死后不同时间和不同脏器中两种看家基因的mRNA降解规律，建立mRNA降解的程度与死亡时间关系的回归方程，探索用于晚期死亡时间推断较为理想的组织，并试图建立标准化的RNA提取与定量检测方法，希望能最终应用于法医学实践中。
延伸阅读
immune PCR分子式：CAS号：性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
Real-time Quantitative PCR (RT-qPCR) and its English Usage Examples 实时荧光定量PCR（RT-FQ-PCR）是一种快速 准确的基因分析技术 通过检测荧光信号实现DNA定量分析。这种技术在医学 生物学和环境科学等领域得到广泛应用。 English Usage Examples: 1. RT-qPCR is a powerful technique for quantifying gene expression levels in real-time. 2. RT-qPCR allows for the accurate measurement of DNA amplification during the PCR process. 3. The results of RT-qPCR provide valuable insights into the dynamics of gene expression under different experimental conditions. 4. RT-qPCR is commonly used to validate the results obtained from RNA sequencing experiments. 5. The high sensitivity of RT-qPCR makes it an essential tool for detecting rare transcripts in biological samples. 如果这些例句不满足你的需求 请告诉我需要哪方面的例句 我可以根据你的需求继续提供。
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